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Moreover, the urinary excretion of isoprostane, a product of the nonenzymatic oxidation of arachidonic acid, and a marker of oxidative stress, was increased in vehicle-treated D 2 / mice and was almost completely normalized by etamicastat treatment (Fig

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Whole blood samples for FA analysis were kept on ice, frozen at 20 C (within 30 min) for 24 h, then stored at 80 C until analysis